Journal: Nutrients

Article Title: Asiatic Acid Prevents the Deleterious Effects of Valproic Acid on Cognition and Hippocampal Cell Proliferation and Survival

doi: 10.3390/nu8050303

Figure Lengend Snippet: The number of Ki-67 positive cells in the SGZ of the hippocampal dentate gyrus. Ki-67 positive cells were stained in the SGZ of the dentate gyrus (green; ( A )). All nuclei were counterstained with propidium iodide (red; ( B ) and figure merged ( C )). The number of proliferating cells in animals receiving only VPA was significantly lower (* p < 0.05) than vehicle, Asiatic acid and Asiatic acid plus VPA groups, while Asiatic acid and Asiatic acid plus VPA groups were significantly higher when compared to the vehicle group (# p < 0.05). One-way ANOVA with LSD post hoc test was used to compare between all groups ( D ).

Article Snippet: Sections ware fixed in 0.5% paraformaldehyde (pH 7.4) for 3 min and then sections were incubated with primary antibody monoclonal mouse Ki-67 (1:150, Vector Laboratory, Inc., Burlingame, CA, USA) at room temperature for 1 h. Sections were then incubated with secondary antibody Alexa 488 rabbit anti mouse IgG (1:300, Invitrogen, Carlsbad, CA, USA) for 40 min and counter stained with propidium iodide (PI) (1:6000, Sigma Aldrich, Inc., St. Louis, MO, USA) for 30 s and mounted in glycerol.

Techniques: Staining

Journal:

Article Title: Puma is required for p53-induced depletion of adult stem cells

doi: 10.1038/ncb2100

Figure Lengend Snippet: Depletion of adult neural stem cells in the dentate gyrus of p53TSD/− mice, which is suppressed by Puma-deficiency. (a) Representative images of sections taken from the brains of 30-day-old p53+/+-, p53+/−-, p53TSD/−-and p53TSD/− Puma−/−-littermates that were immunochemically stained for Ki67, doublecortin and NeuN. (b) Sections from a were immunochemically stained for Sox2 and Glial fibrillary acidic protein (GFAP) to identify the neural stem cells and neurons. Scale bar, 50 μm.

Article Snippet: The primary antibodies used were mouse anti-neuronal-specific nuclear protein (NeuN) antibody (1:200; Chemicon), goat anti-DCX (1:250; Santa Cruz Biotechnology), rabbit anti-Ki67 (1:500; Vector Laboratories), chicken anti-GFAP (1:1000; Chemicon) and rabbit anti-Sox2 (1:200; Chemicon).

Techniques: Staining

Journal: Neural Regeneration Research

Article Title: N-methyl-D-aspartate receptor subunit 1 regulates neurogenesis in the hippocampal dentate gyrus of schizophrenia-like mice

doi: 10.4103/1673-5374.262597

Figure Lengend Snippet: Effect of NMDA on the immunoreactivity of Ki67 in the hippocampal dentate gyrus of a schizophrenia-like mouse. (A) Ki67-immunoreactive cells (green, Alexa Fluor 488 labeled) in the hippocampal dentate gyrus. The arrows point to the Ki67-immunoreactive cells. Scale bars: 40 μm. The right virtual frame (E–H) is an enlarged version of the left virtual frame (A–D). (B) Amount of Ki67-immunoreactive cells in the hippocampal dentate gyrus. The histogram data range is the average number of immunoreactive cells in each slice of each group. All data are shown as the mean ± SD ( n = 10; oneway analysis of variance followed by least significant difference test). * P < 0.05, vs . control group; # P < 0.05, vs . SP or NS group. Con: Control; SP: schizophrenia; NS: normal saline; NMDA: N-methyl-D-aspartate.

Article Snippet: For immunofluorescent staining, brain sections were incubated with a rabbit monoclonal antibody against NR1 (1:500; Abcam, Cambridge, MA, USA), a rat monoclonal antibody against BrdU (1:500; Immunologicals Direct, Oxford Biotechnology Ltd., UK), and a rabbit polyclonal antibody against Ki67 (1:500; Abcam) overnight at 4°C, followed by incubation with an Alexa Fluor 488 labeled goat-anti-rabbit IgG (1:500; Life Technologies Corporation, Gaithersburg, MD, USA) and a Cy3 labeled donkey-anti-rat IgG (1:200; Jackson ImmunoResearch Laboratories, West Grove, PA, USA) for 1 hour at room temperature.

Techniques: Labeling, Control, Saline

Journal: Cancer Research Communications

Article Title: Novel Syngeneic Cell Lines for Studying High-Risk BRAF V600E -Driven Colorectal Cancer In Vivo

doi: 10.1158/2767-9764.CRC-25-0599

Figure Lengend Snippet: All NaJa cell lines successfully engraft into syngeneic immunocompetent C57BL/6N mice and recapitulate their individual 2D morphologies in vivo . A, Schematic overview of the PVI of NaJa cells ( n = 2 NaJa-D, n = 10 NaJa-F, and n = 3 NaJa-G). B, Left, livers showing metastatic nodules 4 weeks after injection (black arrows) and invasive growth in the pancreatic tissue (blue circles). Scale bar, 1 cm. Hematoxylin and eosin: Histology showing that all NaJa cell lines establish tumors (pale blue) in the liver parenchyma (pink) with a differentiation state matching their epithelial or mesenchymal in vitro morphology. Right, IHC and immunofluorescence (IF) staining of proliferation marker Ki-67 (left) and intestinal differentiation markers CDX2 (middle) and E-cadherin (right). L, liver; T, tumor. Scale bar (overview), 500 μm, scale bar (zoom, IHC, and IF), 50 μm. C, Quantification of Ki-67 and CDX2 IHC staining in NaJa cell–induced liver metastases. Each dot represents an individual tumor; different shapes indicate individual tissue sections, and colors denote individual mice. Data are shown as the median ±95% confidence interval (CI).Statistical significance was assessed using the Kruskal–Wallis test followed by Dunn multiple comparison test. **, P < 0.01; ****, P < 0.0001. D, IHC staining of ERK and its downstream target DUSP6. Red arrows indicate examples of nuclear ERK staining. L, liver; T, tumor. Scale bar, 50 μm. [ A, Created by J. Traichel in BioRender. Brummer, T. (2025) https://BioRender.com/8ogg64c .]

Article Snippet: The formalin-fixed, paraffin-embedded (FFPE) sections underwent standard processing and staining procedures using the following primary antibodies directed against E-cadherin (1:50, #610181, BD Laboratories; RRID: AB_397580) and Ki-67 (1:400, #9129, Cell Signaling Technology; RRID: AB_2687446).

Techniques: In Vivo, Injection, In Vitro, Immunofluorescence, Staining, Marker, Immunohistochemistry, Comparison